Search results for: 'Chang'

Q. What are the differences between the CHANG Medium formulations? A. CHANG Medium C, D, In Situ and Amnio are formulated specifically for prenatal cytogenetic testing of amniocytes, CVS (Chorionic Villus Sampling) and POCs (Products of Conception). Q. What is the proper way to reconstitute the CHANG Medium C lyophilized supplement? A. First allow the supplement to equilibrate to room temperature. Once equilibrated, under aseptic conditions the appropriate amount of sterile, distill...

Q. Is it possible to add protein, such as Serum Substitute Supplement (SSS) or Human Serum Albumin to complete media? A. Our complete media contain the concentration of protein indicated below; it is possible to add more protein if needed, as long as you consider that the following has already been added. Multipurpose Handling Media-Complete (MHM-C) with Gentamicin is pre-supplemented with HSA for a final protein concentration of 5 mg/mL. Continuous Single Culture-NX Complete (CSCM-N...

Q. How do we prevent mycoplasma contamination in cell culture? A. Good aseptic techniques should be used in all laboratory applications. The best method for prevention is to test all materials that come in contact with the cells. This should be done on a regular basis to ensure that mycoplasma is not introduced into the cell culture and to identify any potential sources of contamination. Q. Should I be concerned with mycoplasma in my cell culture? A. Mycoplasma contamination should always b...

Q. How long after opening bottles can we use the products? A. When stored as directed, products are stable until the expiration date shown on the bottle. Upon opening the bottles, refer to the product insert for the recommended usage time. Q. Is it ok to aliquot into smaller vials? A. As long as the clinic works in an aseptic, sterile environment, the products can be aliquoted into smaller bottles or vials and stored per the directions. Please note that we cannot guarantee that the aliquots...

Q. Is Human Serum Albumin (HSA) azide-free? A. Yes it is. Azide is commonly used as a preservative for diagnostic grade albumins. Q. Is HSA a blood-based product? A. Yes. HSA consists of human serum albumin from therapeutic-grade source material (100mg/mL) in a saline solution. Q. What kind of oil do you produce? A. We offer both a light and heavy mineral oil intended for use as an overlay during embryo culture to prevent evaporation, changes in osmolality, and pH shifts. ...

Q. What is the glucose concentration in the HEK 293 medium and feed? A. BalanCD HEK 293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. Q. How should adherent HEK 293 cells (banked in a FCS containing medium) be adapted into suspension in BalanCD HEK 293 medium? A. We recommend starting with adapting adherent HEK cells to suspension culture in BalanCD HEK 293 medium with serum (the same concentration the cells have been adapted to). Follow the standard pro...