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CTGrade rh IL-7 is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 97% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
IL-7 promotes the stimulation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. IL-7, along with IL-2, 15, and 21, is a member of the common gamma (γc) chain cytokine family.
Secreted by bone marrow and thymic stromal cells, dendritic cells, intestinal epithelial cells, hepatocytes, and keratinocytes
Signals through its receptor (IL-7R) to promote the differentiation of hematopoietic stem cells into T cells, B cells, and natural killer cells
Is a regulator of intestinal mucosal lymphocyte proliferation
Promotes favorable phenotypes in adoptive cellular therapies such as CAR-T cell therapy when combined with IL-15. The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Prominent
Band at 17.5 kDa
Figure 1. CTGrade rh IL-7 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a prominent band at 17.5 kDa.
Cell-Based Proliferation Assay
Figure 2. The biological activity of CTGrade rh IL-7 was determined in a PHA-stimulated human PBMC cell-based proliferation assay.
CTGrade rh IL-7 lyophilized at 1 mg/ml in 20 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered
Protein Purity
≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin
< 0.05 EU/µg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent proliferation assay using PHA-activated PBMC. The ED50 is typically less than 0.5 ng/mL.
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
*Animal component-free to the tertiary level (no animal components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.
**For research use or further manufacturing and in ex vivo cell therapy applications.
***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
