Recombinant Rat TNF-α

In stock

Catalog ID

300-18

Size	Stock	SKU	Unit Price
5 µg	In Stock	300-18-5UG	$73.00
20 µg	In Stock	300-18-20UG	$183.00
100 µg	In Stock	300-18-100UG	$457.00
500 µg	Inquire for Availability	300-18-500UG	$1,971.00
1 mg	Inquire for Availability	300-18-1MG	$3,284.00

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Details

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF
Accession Number	P16599
Source	Genetically modified E.coli
Predicted Molecular Mass	Monomer, 17.3 kDa (157 aa)
AA Sequence	MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Formulation	Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 1 EU/µg
Biological Activity (ED₅₀)	Cytolysis of mouse L929 cells in the presence of Actinomycin D	≤ 0.05 ng/mL

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to-80°C as supplied.

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