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Recombinant Rat IL-3 β
Interleukin 3 beta (IL-3 β) is a cytokine that is produced by activated T cells and mast cells. IL-3 β induces the differentiation of hematopoietic stem cells into myeloid precursor cells, such as erythrocyte, megakaryocyte, granulocyte, monocyte, and dendritic cells. IL-3 β also functions in the nervous system and is important during the B-1 cell regulation of chronic inflammatory diseases.
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Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
Alternate Names | MCGF, Multi-CSF, HCGF, P-cell stimulating factor |
Accession Number | P04823 |
Source | Genetically modified E.coli |
Predicted Molecular Mass | Monomer, 16.3 kDa (144 aa) |
AA Sequence | MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC |
Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
Product Reconstitution | Sterile water at 0.1 mg/mL |
Country of Origin | USA |
Specification* | Method of Determination | Acceptance Criteria |
Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
Biological Activity (ED50) | NFS-60 cell proliferation | ≤ 10 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.