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Recombinant Rat GM-CSF
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.
Technical Specifications
Alternate Names | CSF-2, Pluripoietin-α, MGI1GM |
Accession Number | P48750 |
Source | Genetically modified E.coli |
Predicted Molecular Mass | Monomer, 14.7 kDa (128 aa) |
AA Sequence | MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK |
Formulation | Lyophilized from a 0.2 micron filtered solution containing 20 mM sodium bicarbonate, pH 8.5 |
Product Reconstitution | Sterile water at 0.1 mg/mL |
Country of Origin | USA |
Specification* | Method of Determination | Acceptance Criteria |
Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
Biological Activity (ED50) | FDC-P1 cell proliferation | ≤ 0.02 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.