Recombinant Rat GM-CSF

In stock

Catalog ID

300-03

Size	Stock	SKU	Unit Price
5 µg	In Stock	300-03-5UG	$73.00
20 µg	In Stock	300-03-20UG	$183.00
100 µg	In Stock	300-03-100UG	$457.00
500 µg	In Stock	300-03-500UG	$2,045.00
1 mg	In Stock	300-03-1MG	$3,406.00

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Details

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	CSF-2, Pluripoietin-α, MGI1GM
Accession Number	P48750
Source	Genetically modified E.coli
Predicted Molecular Mass	Monomer, 14.7 kDa (128 aa)
AA Sequence	MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK
Formulation	Lyophilized from a 0.2 micron filtered solution containing 20 mM sodium bicarbonate, pH 8.5
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	FDC-P1 cell proliferation	≤ 0.02 ng/mL

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20 to -80 ºC as supplied.

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