Recombinant Human VEGF-165

In stock

Catalog ID

100-44

Size	Stock	SKU	Unit Price
10 µg	In Stock	100-44-10UG	$183.00
100 µg	In Stock	100-44-100UG	$529.00
500 µg	In Stock	100-44-500UG	$2,627.00
1 mg	In Stock	100-44-1MG	$4,379.00
10 µg (Animal-Free)	In Stock	100-44AF-10UG	$200.00
100 µg (Animal-Free)	In Stock	100-44AF-100UG	$577.00
500 µg (Animal-Free)	Inquire for Availability	100-44AF-500UG	$2,889.00
1 mg (Animal-Free)	In Stock	100-44AF-1MG	$4,817.00

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Details

Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	VEGF-A, VPF, glioma-derived endothelial cell mitogen
Accession Number	P15692-4
Source	Genetically modified E.coli
Predicted Molecular Mass	Dimer, 19.3/38.6 kDa (166/332 aa)
AA Sequence	MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Formulation	Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	HUVEC proliferation	≤ 10 ng/mL

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to-80°C as supplied.

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