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Recombinant Human VEGF-165

In stock
Catalog ID
100-44
Size Stock SKU Unit Price Qty
10 µg In Stock 100-44-10UG
$183.00
10 µg (Animal-Free) In Stock 100-44AF-10UG
$200.00
100 µg In Stock 100-44-100UG
$529.00
100 µg (Animal-Free) In Stock 100-44AF-100UG
$577.00
500 µg In Stock 100-44-500UG
$2,627.00
500 µg (Animal-Free) Inquire for Availability 100-44AF-500UG
$2,889.00
1 mg In Stock 100-44-1MG
$4,379.00
1 mg (Animal-Free) In Stock 100-44AF-1MG
$4,817.00
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Details

Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names VEGF-A, VPF, glioma-derived endothelial cell mitogen
Accession Number P15692-4
Source Genetically modified E.coli
Predicted Molecular Mass Dimer, 19.3/38.6 kDa (166/332 aa)
AA Sequence MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Formulation Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Product Reconstitution Sterile water at 0.1 mg/mL
Country of Origin USA

 

Specification* Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
Endotoxin Kinetic LAL ≤ 0.1 EU/µg
Biological Activity (ED50) HUVEC proliferation ≤ 10 ng/mL

 

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20°C to-80°C as supplied.
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