Recombinant Human PDGF-BB

In stock

Catalog ID

100-18

Size	Stock	SKU	Unit Price
10 µg	In Stock	100-18-10UG	$183.00
100 µg	In Stock	100-18-100UG	$517.00
500 µg	In Stock	100-18-500UG	$2,554.00
1 mg	In Stock	100-18-1MG	$4,258.00
10 µg (Animal-Free)	In Stock	100-18AF-10UG	$200.00
100 µg (Animal-Free)	In Stock	100-18AF-100UG	$566.00
500 µg (Animal-Free)	In Stock	100-18AF-500UG	$2,811.00
1 mg (Animal-Free)	In Stock	100-18AF-1MG	$4,683.00

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Details

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β, and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	GDGF, ODGF
Accession Number	P01127
Source	Genetically modified E.coli
Predicted Molecular Mass	Dimer, 12.4/24.9 kDa (110/220 aa)
AA Sequence	MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Formulation	Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	NR6R-3T3 cell proliferation	≤ 20 ng/mL; ≥ 5.0 x 10⁴ units/mg

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to -80°C as supplied.

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