Recombinant Human / Mouse / Rat Activin-A
Activin A is a member of the transforming growth factor beta (TGF-β) family of proteins and functions to stimulate follicle-stimulating hormone (FSH) secretion. Activins are produced in many tissue types including the skin, gonads, lungs, and pituitary gland. Activins interact with receptor type I and type II serine/threonine protein kinases, to activate SMAD signaling and regulate diverse cellular functions, such as cell proliferation, differentiation, wound healing, apoptosis, and metabolism. Activin A is a homodimer comprised of two activin βA chains. Cleavage of the N-terminal propeptide renders the Activin protein biologically active. Human Activin A shares 100% amino acid sequence identity with mouse, rat, porcine, bovine, and feline Activin A proteins.
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Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
|Alternate Names||Inhibin beta-1, FRP, FSH-releasing protein|
|Source||Genetically modified E.coli|
|Predicted Molecular Mass||Dimer, 13.1/26.2 kDa (117/234 aa)|
|AA Sequence||MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS|
|Formulation||Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Product Reconstitution||Sterile water at 0.1 mg/mL|
|Country of Origin||USA|
|Specification*||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS PAGE||≥ 95%|
|Endotoxin||Kinetic LAL||≤ 0.1 EU/µg|
|Biological Activity (ED50)||Cytotoxicity of MPC-11 cells||≤ 10 ng/mL|
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.