Recombinant Human IL-6
Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells.
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Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
|Alternate Names||IFN-β2, B-cell Differentiation Factor, BSF-2, HSF, MGI-2|
|Source||Genetically modified E.coli|
|Predicted Molecular Mass||Monomer, 21 kDa (185 aa)|
|AA Sequence||MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM|
|Formulation||Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5|
|Product Reconstitution||Sterile water at 0.1 mg/mL|
|Country of Origin||USA|
|Specification*||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS PAGE||≥ 97%|
|Endotoxin||Kinetic LAL||≤ 0.1 EU/µg|
|Biological Activity (ED50)||B9 cell proliferation||≤ 0.025 ng/mL; ≥ 4.0 x 107 units/mg|
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.