CTGrade rh IL-3
CTGrade rh IL-3 is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:
- High biological activity verified by a relevant bioactivity assay
- Low endotoxin levels
- ≥ 97% purity
- High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
IL-3 is a species-specific cytokine and pleiotropic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages.
- Produced by T cells, mast cells, and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity
- Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles
- Many of the IL-3 activities depend upon co-stimulation with other cytokines
The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade products are manufactured in a facility that does not use or process beta lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Single Prominent Band at 15.2 kDa
Figure 1. CTGrade rh IL-3 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 15.2 kDa.
Cell-Based Proliferation Assay
Figure 2. The biological activity of CTGrade rh IL-3 was determined in cell proliferation assay using TF-1 erythroleukamic cells.
|Alternate Names||MCGF, Multi-CSF, HCGF, P-cell stimulating factor|
|AA Sequence||MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF|
|Predicted Molecular Mass||Monomer, 15.2 kDa (134aa)|
|Formulation||CTGrade rh IL-3 lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, 150 mM Sodium Chloride, pH 7.5, 0.2 µm filtered|
|Protein Purity||≥ 97% determined by reducing and non-reducing SDS-PAGE analysis|
|Endotoxin||< 0.05 EU/µg using USP <85> / EP 2.6.14|
|Bioactivity||ED50 is determined by the dose-dependent Proliferation of TF-1 cells. The ED50 is typically less than 2 ng/mL. The international units of CTGrade rh IL-3 is approximately 4.2 x103 IU/µg, which is calibrated against recombinant Human Interleukin 3 WHO International Standard (NIBSC code 91/510).|
|Quality||Carrier-free and no animal- or human-derived materials were used during manufacturing|
*For research use or further manufacturing and in ex vivo cell therapy applications.
**Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.
***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.