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Recombinant Mouse IL-12

In stock
Catalog ID
200-25
Size Stock SKU Unit Price Qty
10 µg Inquire for Availability 200-25-10UG
$183.00
    100 µg Inquire for Availability 200-25-100UG
    $1,460.00
      500 µg Inquire for Availability 200-25-500UG
      $5,255.00
        1 mg Inquire for Availability 200-25-1MG
        $8,759.00
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          Details

          Interleukin 12 (IL-12) is an inducer of cell-mediated immunity and is produced by dendritic cells, macrophages, and B cells after antigen stimulation. IL-12 functions to promote naïve T cell differentiation into the type 1 T helper cell (Th1) lineage. IL-12 also stimulates the production of interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) from T cells and natural killer cells. Mouse IL-12 is active on human and mouse cells.

          Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

          Instructions

          Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

          Technical Specifications

          Alternate Names NKSF, CLMF
          Accession Number P43432/P43431
          Source Genetically modified HEK 293 cells
          Predicted Molecular Mass Heterodimer, p35: 21.7 kDa, p40: 35.8 kDa, total: 57.5 kDa (p35: 193 aa, p40: 313 aa, total: 506 aa)
          AA Sequence p35: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

          p40: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
          Formulation Lyophilized from a sterile 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5
          Product Reconstitution Sterile water at 0.1 mg/mL
          Country of Origin USA

           

          Specification* Method of Determination Acceptance Criteria
          Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
          Endotoxin Kinetic LAL ≤ 0.1 EU/µg
          Biological Activity (ED50) Induces secretion of SEAP in HEK-Blue IL-12 cells ≤ 15 ng/mL; 6.7 x 104 units/mg

           

          *Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

          Specifications

          Storage
          At or below -20°C
          Shelf Life
          12 months from date of receipt when stored at -20°C to-80°C as supplied.
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