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Recombinant Human IL-1α / IL-1F1
In stock
Catalog ID
100-173
Details
Interleukin 1 alpha (IL-1α) is expressed by epithelial cells, activated macrophages, neutrophils, and endothelial cells to regulate immune responses. IL-1α signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1α and the independently regulated IL-1β protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | IL-1F1, FAF, BAF, LEM, LAF |
| Accession Number | P01583 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 18 kDa (159 aa) |
| AA Sequence | SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 1 EU/µg |
| Biological Activity (ED50) | D10.G4.1 cell proliferation | ≤ 0.05 ng/mL; ≥ 2.0 x 107 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
Specifications
Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20°C to-80°C as supplied.
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