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Recombinant Human FGF-basic 154 / FGF-2

In stock
Catalog ID
100-146
Size Stock SKU Unit Price Qty
10 µg Inquire for Availability 100-146-10UG
$73.00
    50 µg Inquire for Availability 100-146-50UG
    $183.00
      100 µg In Stock 100-146-100UG
      $262.00
        500 µg In Stock 100-146-500UG
        $560.00
          1 mg Inquire for Availability 100-146-1MG
          $973.00
            10 µg (Animal-Free) In Stock 100-146AF-10UG
            $79.00
              50 µg (Animal-Free) In Stock 100-146AF-50UG
              $200.00
                100 µg (Animal-Free) In Stock 100-146AF-100UG
                $286.00
                  500 µg (Animal-Free) In Stock 100-146AF-500UG
                  $614.00
                    1 mg (Animal-Free) Inquire for Availability 100-146AF-1MG
                    $1,035.00
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                      Details

                      Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene. There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.

                      Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

                      Instructions

                      Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

                      Technical Specifications

                      Alternate Names FGF2, HBGF-2, Prostatropin
                      Accession Number P09038
                      Source Genetically modified E.coli
                      Predicted Molecular Mass Monomer, 17.3 kDa (155 aa)
                      AA Sequence MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
                      Formulation Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
                      Product Reconstitution Sterile water at 0.1 mg/mL
                      Country of Origin USA

                       

                      Specification* Method of Determination Acceptance Criteria
                      Purity Reducing and Non-Reducing SDS-PAGE ≥ 95%
                      Endotoxin Kinetic LAL ≤ 0.1 EU/µg
                      Biological Activity (ED50) NR6R-3T3 Proliferation ≤ 5 ng/mL; ≥ 2.0 x 105 units/mg

                       

                      *Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

                      Specifications

                      Storage
                      At or below -20°C
                      Shelf Life
                      12 months from date of receipt when stored at -20°C to -80°C as supplied.
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