Recombinant Human FGF-basic 154 / FGF-2

In stock

Catalog ID

100-146

Size	Stock	SKU	Unit Price
10 µg	In Stock	100-146-10UG	$73.00
50 µg	Inquire for Availability	100-146-50UG	$183.00
100 µg	In Stock	100-146-100UG	$262.00
500 µg	In Stock	100-146-500UG	$560.00
1 mg	Inquire for Availability	100-146-1MG	$973.00
10 µg (Animal-Free)	In Stock	100-146AF-10UG	$79.00
50 µg (Animal-Free)	In Stock	100-146AF-50UG	$200.00
100 µg (Animal-Free)	In Stock	100-146AF-100UG	$286.00
500 µg (Animal-Free)	In Stock	100-146AF-500UG	$614.00
1 mg (Animal-Free)	In Stock	100-146AF-1MG	$1,035.00

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Details

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene. There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	FGF2, HBGF-2, Prostatropin
Accession Number	P09038
Source	Genetically modified E.coli
Predicted Molecular Mass	Monomer, 17.3 kDa (155 aa)
AA Sequence	MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Formulation	Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS-PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	NR6R-3T3 Proliferation	≤ 5 ng/mL; ≥ 2.0 x 10⁵ units/mg

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to -80°C as supplied.

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