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Recombinant Human FGF-acidic / FGF-1
Acidic fibroblast growth factor (FGF-acidic), also known as FGF-1, is a potent inducer of DNA synthesis, cell proliferation, and has chemotactic activities. FGF-acidic regulates cardiogenesis through protein kinase C signaling. FGF-acidic also functions as an insulin sensitizer and mediates adipose tissue remodeling. High serum levels of FGF-acidic are associated with type 2 diabetes mellitus (T2DM), suggesting a pathogenic role of FGF-acidic during T2DM.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
Technical Specifications
Alternate Names | FGF1, HBGF-1, Beta-endothelial growth factor, ECGF-beta |
Accession Number | P05230 |
Source | Genetically modified E.coli |
Predicted Molecular Mass | Monomer, 16 kDa (141 aa) |
AA Sequence | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 150 mM sodium sulfate, pH 7.5 |
Product Reconstitution | Sterile water at 0.1 mg/mL |
Country of Origin | USA |
Specification* | Method of Determination | Acceptance Criteria |
Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
Biological Activity (ED50) | NR6R-3T3 cell proliferation | ≤ 2 ng/mL; ≥ 5.0 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.