Join Our Mailing List Today!

NOTICE: Online ordering will be disabled 1/27-2/3. Learn More.

Recombinant Human BMP-4

In stock
Catalog ID
100-212
Size Stock SKU Unit Price Qty
5 µg In Stock 100-212-5UG
$183.00
    20 µg In Stock 100-212-20UG
    $584.00
      100 µg In Stock 100-212-100UG
      $2,045.00
        500 µg In Stock 100-212-500UG
        $3,643.00
          1 mg In Stock 100-212-1MG
          $6,076.00
            reset
            Details

            Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic sub-family (BMP) and functions as a critical element involved in the culture and differentiation of stem cells. Active BMP-4 consists of forming a homodimer or a heterodimer with related BMPs, such as BMP-7. BMP-4 is integral in stem cell cultures and the differentiation of mesoderm and ectoderm from pluripotent stem cells (iPSCs and ESCs). BMP-4 is used in protocols for culturing cardiomyocytes, early hematopoietic cells, hepatocytes, bone, cartilage, adipocytes and lung, as well as, inhibition of early neuronal differentiation.

            Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

            Instructions

            Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.   

            Technical Specifications

            Alternate Names BMP-2B
            Accession Number P12644
            Source Genetically modified CHO cells
            Predicted Molecular Mass Dimer, 13.1/26.2 kDa (116/232 aa)
            AA Sequence SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAI VQT LVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
            Formulation Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
            Product Reconstitution Sterile 10 mM HCl at 0.1 mg/mL
            Country of Origin USA

             

            Specification* Method of Determination Acceptance Criteria
            Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
            Endotoxin Kinetic LAL (50% confidence) ≤ 0.1 EU/µg
            Biological Activity (ED50) Alkaline phosphatase activity induced in ATDC-5 cells ≤ 15 ng/mL

             

            *Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

            Specifications

            Storage
            At or below -20°C
            Shelf Life
            12 months from date of receipt when stored at -20°C to-80°C as supplied.
            © 2024 FUJIFILM Irvine Scientific. All rights reserved.