Recombinant Human BMP-4

In stock

Catalog ID

100-212

Size	Stock	SKU	Unit Price
5 µg	In Stock	100-212-5UG	$183.00
20 µg	In Stock	100-212-20UG	$584.00
100 µg	In Stock	100-212-100UG	$2,045.00
500 µg	Inquire for Availability	100-212-500UG	$3,643.00
1 mg	Inquire for Availability	100-212-1MG	$6,076.00

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Details

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic sub-family (BMP) and functions as a critical element involved in the culture and differentiation of stem cells. Active BMP-4 consists of forming a homodimer or a heterodimer with related BMPs, such as BMP-7. BMP-4 is integral in stem cell cultures and the differentiation of mesoderm and ectoderm from pluripotent stem cells (iPSCs and ESCs). BMP-4 is used in protocols for culturing cardiomyocytes, early hematopoietic cells, hepatocytes, bone, cartilage, adipocytes and lung, as well as, inhibition of early neuronal differentiation.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	BMP-2B
Accession Number	P12644
Source	Genetically modified CHO cells
Predicted Molecular Mass	Dimer, 13.1/26.2 kDa (116/232 aa)
AA Sequence	SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAI VQT LVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
Formulation	Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Product Reconstitution	Sterile 10 mM HCl at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL (50% confidence)	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	Alkaline phosphatase activity induced in ATDC-5 cells	≤ 15 ng/mL

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to-80°C as supplied.

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