Recombinant Human β-NGF

In stock

Catalog ID

100-38

Size	Stock	SKU	Unit Price
5 µg	In Stock	100-38-5UG	$73.00
20 µg	In Stock	100-38-20UG	$183.00
100 µg	In Stock	100-38-100UG	$457.00
500 µg	Inquire for Availability	100-38-500UG	$1,971.00
1 mg	In Stock	100-38-1MG	$3,284.00
5 µg (Animal-Free)	In Stock	100-38AF-5UG	$79.00
20 µg (Animal-Free)	In Stock	100-38AF-20UG	$200.00
100 µg (Animal-Free)	In Stock	100-38AF-100UG	$504.00
500 µg (Animal-Free)	In Stock	100-38AF-500UG	$2,165.00
1 mg (Animal-Free)	In Stock	100-38AF-1MG	$3,613.00

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Details

Nerve growth factor beta (β-NGF) is a neurotrophic factor that is important for the development and maintenance of sensory and sympathetic neurons. β-NGF signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways. β-NGF is also involved in the growth, differentiation, and survival of B lymphocytes. Human, mouse, and rat β-NGF proteins are cross-reactive.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	beta-NGF
Accession Number	P01138
Source	Genetically modified E.coli
Predicted Molecular Mass	Noncovalent Homodimer, 13.6 kDa (121 aa)
AA Sequence	MSSSHPIFHR GEFSVCDSVS VWVGDKTTAT DIKGKEVMVL GEVNINNSVF KQYFFETKCR DPNPVDSGCR GIDSKHWNSY CTTTHTFVKA LTMDGKQAAW RFIRIDTACV CVLSRKAVRR A
Formulation	Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	TF-1 cell proliferation	≤ 5 ng/mL

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to-80°C as supplied.

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