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Recombinant Human ACRP-30
The globular subunit of adipocyte complement-related protein of 30 kDa (ACRP-30) is a naturally occurring cleavage product of adiponectin, a protein made exclusively by adipocytes. ACRP-30 is an abundant serum protein and plays an important role in hyperglycemia, insulin resistance, and fatty acid oxidation. ACRP-30 signals through adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2).
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
Alternate Names | Adiponectin, apm-1, adipolean |
Accession Number | Q15848 |
Source | Genetically modified E.coli |
Predicted Molecular Mass | Monomer, 16.7 kDa (145 aa) |
AA Sequence | MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN |
Formulation | Lyophilized from a 0.2 µm filtered aqueous solution containing 10 mM sodium phosphate, 0.5 mM DTT, pH 7.5 |
Product Reconstitution | Sterile 10 mM sodium phosphate, 0.5 mM DTT, pH 7.5 at 0.1 mg/mL |
Country of Origin | USA |
Specification* | Method of Determination | Acceptance Criteria |
Purity | Reducing and Non-Reducing SDS PAGE | ≥ 90% |
Endotoxin | Kinetic LAL | ≤ 1 EU/µg |
Biological Activity (ED50) | Inhibition of M1 Cell Proliferation | ≤ 2,000 ng/mL; ≥ 500 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.