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CTGrade rh FLT-3 Ligand

In stock
Catalog ID
500-03
Size Stock SKU Unit Price Qty
50 µg In Stock 500-03-50UG
$1,196.00
100 µg In Stock 500-03-100UG
$1,720.00
1 mg Inquire for Availability 500-03-1MG
$7,372.00
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Details

CTGrade rh FLT-3 Ligand is a recombinant protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:

  • High biological activity verified by a relevant bioactivity assay
  • Low endotoxin levels
  • ≥ 97% purity
  • High lot-to-lot consistency

Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.

FLT-3 Ligand is a growth factor that regulates proliferation of early hematopoietic cells. FLT-3 Ligand binds to cells expressing the tyrosine kinase receptor FLT-3.

  • FLT-3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation
  • Unlike SCF, FLT-3 Ligand exerts no activity on mast cells. Multiple isoforms of FLT-3 Ligand have been identified
  • The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 aa)
  • The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform

The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

The CTGrade products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.

SDS-PAGE Showing a Single Prominent Band at 17.7 kDa

Figure 1. CTGrade rh FLT-3 Ligand (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 17.7 kDa.

Cell-Based Proliferation Assay

Figure 2. The biological activity of CTGrade rh FLT-3 Ligand was determined in a cell proliferation assay in OCI-AML5 cells.

Technical Specifications

Alternate Names Flt3L, Fms-like tyrosine kinase 3 ligand
Accession Number P49771
AA Sequence MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT
Predicted Molecular Mass Monomer, 17.7 kDa (155aa)
Formulation CTGrade rh FLT-3 Ligand lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5, 0.2 µm filtered
Protein Purity ≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin < 0.05 EU/µg using USP <85>/EP 2.6.14
Bioactivity ED50 is determined by the dose-dependent proliferation of OCI-AML5 cells. The ED50 is typically less than 5 ng/mL. The international units of CTGrade rh FLT-3 Ligand is approximately 1.6 x 103 IU/µg, which is calibrated against recombinant human FLT-3 ligand WHO International Standard (NIBSC code 96/532).
Quality Carrier-free and no animal- or human-derived materials were used during manufacturing

 

*For research use or further manufacturing and in ex vivo cell therapy applications.

**Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.

***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
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