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CTGrade GMP rh IL-6 is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 97% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
IL-6 is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages, and muscle cells.
Signals through a receptor complex containing two receptors, IL-6Rα and gp130
Plays an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta- lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE
Showing a Single Prominent Band at 20.9 kDa
Figure 1. CTGrade GMP rh IL-6 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) conditions and visualized by InstantBlue staining showing a single prominent band at 20.9 kDa.
Cell-Based
Proliferation Assay
Figure 2. The biological activity of CTGrade GMP rh IL-6 was determined by its ability to stimulate the proliferation of B9 cells.
CTGrade GMP rh IL-6 lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered
Protein Purity
≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin
< 0.05 EU/µg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent proliferation of B9 cells. The ED50 is typically less than 0.025 ng/mL. The international units of CTGrade GMP rh IL-6 is approximately 1.2 x105 IU/µg, which is calibrated against recombinant human interleukin 6 WHO International Standard (NIBSC code 89/548).
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
*For research use or further manufacturing and in ex vivo cell therapy applications.
**Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.
***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
