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CTGrade GMP rh IL-4 is a recombinant human protein that is produced from E. coli and is designed to support pre-clinical and clinical research, as well as commercial applications, and offers:
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 98% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
IL-4 is an immunomodulatory cytokine that stimulates B cell proliferation and activates eosinophils, basophils, and mast cells.
Induces vascular cell adhesion molecule (VCAM)-1 expression on endothelial cells and involved in collagen production by fibroblasts
Plays a key role in promotion of Th2 differentiation from naïve helper T cells
Defines the Th2 phenotype of lymphocytes
Regulates cell proliferation, apoptosis, and expression of numerous genes in various cell types
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Prominent Band at 14.4 kDa
Figure 1. CTGrade GMP rh IL-4 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14.4 kDa.*
*Predicted MW is 15.1 kDa, but runs on SDS-PAGE at 14.4 kDa.
Cell-Based Proliferation Assay
Figure 2. The biological activity of CTGrade GMP rh IL-4 was determined in TF-1 cell-based proliferation assay.
CTGrade GMP rh IL-4 lyophilized at 1 mg/ml in 20 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered
Protein Purity
≥ 98% determined with Coomassie staining
Endotoxin
< 0.05 EU/µg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent TF-1 cell proliferation cells. The ED50 is typically less than 0.25 ng/mL. The international units of CTGrade GMP rh IL-4 is approximately 1.4 x 104 IU/µg, which is calibrated against recombinant human interleukin 4 WHO International Standard (NIBSC code 88/656).
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
