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CTGrade GMP rh IL-3

In stock
Catalog ID
500-04
Size Stock SKU Unit Price Qty
50 µg In Stock 500-04-50UG
$1,032.00
    100 µg In Stock 500-04-100UG
    $1,513.00
      1 mg Inquire for Availability 500-04-1MG
      $7,372.00
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        Details

        CTGrade GMP rh IL-3 is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:

        • High biological activity verified by a relevant bioactivity assay
        • Low endotoxin levels
        • ≥ 97% purity
        • High lot-to-lot consistency

        Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.

        IL-3 is a species-specific cytokine and pleiotropic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages.

        • Produced by T cells, mast cells, and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity
        • Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles
        • Many of the IL-3 activities depend upon co-stimulation with other cytokines

        The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

        The CTGrade GMP products are manufactured in a facility that does not use or process beta lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.

        SDS-PAGE Showing a Single Prominent Band at 15.2 kDa 

        Figure 1. CTGrade GMP rh IL-3 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 15.2 kDa.

        Cell-Based Proliferation Assay

        Figure 2. The biological activity of CTGrade GMP rh IL-3 was determined in cell proliferation assay using TF-1 erythroleukamic cells.  

        Technical Specifications

        Alternate Names MCGF, Multi-CSF, HCGF, P-cell stimulating factor
        Accession Number P08700
        AA Sequence MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
        Predicted Molecular Mass Monomer, 15.2 kDa (134aa)
        Formulation CTGrade GMP rh IL-3 lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, 150 mM Sodium Chloride, pH 7.5, 0.2 µm filtered
        Protein Purity ≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
        Endotoxin < 0.05 EU/µg using USP <85> / EP 2.6.14
        Bioactivity ED50 is determined by the dose-dependent proliferation of TF-1 cells. The ED50 is typically less than 2 ng/mL. The international units of CTGrade GMP rh IL-3 is approximately 4.2 x103 IU/µg, which is calibrated against recombinant human interleukin 3 WHO International Standard (NIBSC code 91/510).
        Quality Carrier-free and no animal- or human-derived materials were used during manufacturing

         

        *For research use or further manufacturing and in ex vivo cell therapy applications.

        **Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.

        ***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

        Specifications

        Storage
        This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
        Shelf Life
        Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
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