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Search results for: 'cell media for T'
- Q. What is the density and density gradient of ISolate and ISolate Stock Solution ? A. Here are the density and density gradients of ISolate. Q. Is it acceptable to put TYB in the uterus at insemination? Should TYB be washed prior to IUI? A. We recommend that the TYB freezing medium should be removed prior to IUI either by simple washing with Sperm Washing Medium or Multipurpose Handling Medium - Complete, or if washing thawed raw semen, by density gradient centrifugation with ISolate ...
- Q. What are the differences between the CHANG Medium formulations? A. CHANG Medium C, D, In Situ and Amnio are formulated specifically for prenatal cytogenetic testing of amniocytes, CVS (Chorionic Villus Sampling) and POCs (Products of Conception). Q. What is the proper way to reconstitute the CHANG Medium C lyophilized supplement? A. First allow the supplement to equilibrate to room temperature. Once equilibrated, under aseptic conditions the appropriate amount of sterile, distill...
- Q. Is additional L-glutamine or other supplementation required? A. IS Sf Insect is a complete, ready-to-use medium. Additional supplementation is not required. However, it can be further supplemented according to user needs. Q. What is the L-glutamine concentration in IS Sf Insect? A. The medium contains 8 mM L-Glutamine. Q. What is the glucose level in IS Sf Insect? A. IS Sf Insect contains 8 gm/L glucose. Q. Does the medium contain a sodium bicarbonate or other buffering s...
- Q. Is it possible to add protein, such as Serum Substitute Supplement (SSS) or Human Serum Albumin to complete media? A. Our complete media contain the concentration of protein indicated below; it is possible to add more protein if needed, as long as you consider that the following has already been added. Multipurpose Handling Media-Complete (MHM-C) with Gentamicin is pre-supplemented with HSA for a final protein concentration of 5 mg/mL. Continuous Single Culture-NX Complete (CSCM-N...
- Very Rapid Development Of A Chemically-Defined Feed Medium For A Specific Cell Line Using Spent Media Analysis
- Development of Serum-Free Media for Mesenchymal Stem Cells, Complied with Japanese Standards for Biological Ingredients
- A Versatile and Scalable Media Platform Designed for Cell Based Applications Using Human Embryo
- CHO Media Platform Facilitates Integrated Cell Line Development and Media Optimization
- Development of Next Generation Feed Medium to Support High Performance CHO Cell Lines
- Modulating Antibody Galactosylation through Cell Culture Medium for Improved Function and Product Quality
- Using Protein Hydrolysates as Raw Materials for Serum-Free Cell Culture Media
- Cryopreservation Protocol for PBMCs and T Cells Using PRIME-XV FreezIS DMSO-Free Cryopreservation Medium
- Cryopreservation Media Solutions for Cell and Gene Therapy Development and Manufacturing
- A CHO Media Platform to Facilitate Cell Line Development
- Methods of Rational Culture Media Design and Optimization for a Hybridoma and a Myeloma Cell Line
- Very Rapid Feed Medium Development for Three Different CHO Cell Lines
- Development of a Chemically Defined Growth Medium Specifically Designed for CHO DG44 Cells
- PRIME-XV Media and Cytokines for the Expansion and Differentiation of Hematopoietic Progenitor Cells
- Development of a Serum-Free, Chemically Defined Cell Culture Medium for the Production of HIV from HUT-78 Cells
- Development of a Serum-Free, Chemically Defined Cell Culture Medium for the Production of HIV from HUT-78 Cells
- Using Protein Hydrolysates as Raw Materials for Serum-Free Cell Culture Media
- Q. Why was L-Glutamine eliminated from many of your formulas? A. L-Glutamine is easily hydrolyzed in liquid and byproducts of hydrolysis can be toxic to the cell. Additionally, the necessary concentration of L-Glutamine may be compromised after a period of time. Since L-Glutamine is an essential amino acid, it is best for the end users to supplement media with the recommended concentration of fresh L-Glutamine . Q. What effects could freezing and thawing have on my sera? A. We generally di...
- Q. What is the glucose concentration in the HEK 293 medium and feed? A. BalanCD HEK 293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. Q. How should adherent HEK 293 cells (banked in a FCS containing medium) be adapted into suspension in BalanCD HEK 293 medium? A. We recommend starting with adapting adherent HEK cells to suspension culture in BalanCD HEK 293 medium with serum (the same concentration the cells have been adapted to). Follow the standard pro...
- Q. How does FUJIFILM Irvine Scientific define serum-free, animal-component free, protein-free, and chemically-defined media? A. Here is how FUJIFILM Irvine Scientific defines these types of media: Serum-free media do not contain or require the addition of serum. Protein-free media do not contain any proteins but can contain undefined peptides from plant hydrolysates. FUJIFILM Irvine Scientific animal-component free products are manufactured with raw materials that do not contain any anim...