Search results for: 'custom'

Q. What is the timeline on a custom formula? A. Customs are usually delivered to the customer between 6 to 8 weeks after we receive the final signed documents with a purchase order. Q. What are the minimum requirements for a custom media formula? A. We generally require a 100L minimum order to manufacture a custom formula. Our Express Media Service can make smaller non-GMP quantities to meet special investigational or development needs for our customers. Q. Can you customize my serum...

Q. Why was L-Glutamine eliminated from many of your formulas? A. L-Glutamine is easily hydrolyzed in liquid and byproducts of hydrolysis can be toxic to the cell. Additionally, the necessary concentration of L-Glutamine may be compromised after a period of time. Since L-Glutamine is an essential amino acid, it is best for the end users to supplement media with the recommended concentration of fresh L-Glutamine . Q. What effects could freezing and thawing have on my sera? A. We generally di...

Q. How does FUJIFILM Irvine Scientific define serum-free, animal-component free, protein-free, and chemically-defined media? A. Here is how FUJIFILM Irvine Scientific defines these types of media: Serum-free media do not contain or require the addition of serum. Protein-free media do not contain any proteins but can contain undefined peptides from plant hydrolysates. FUJIFILM Irvine Scientific animal-component free products are manufactured with raw materials that do not contain any anim...

Q. What is the glucose concentration in the HEK 293 medium and feed? A. BalanCD HEK 293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. Q. How should adherent HEK 293 cells (banked in a FCS containing medium) be adapted into suspension in BalanCD HEK 293 medium? A. We recommend starting with adapting adherent HEK cells to suspension culture in BalanCD HEK 293 medium with serum (the same concentration the cells have been adapted to). Follow the standard pro...

Q. What is the density and density gradient of ISolate and ISolate Stock Solution ? A. Here are the density and density gradients of ISolate. Q. Is it acceptable to put TYB in the uterus at insemination? Should TYB be washed prior to IUI? A. We recommend that the TYB freezing medium should be removed prior to IUI either by simple washing with Sperm Washing Medium or Multipurpose Handling Medium - Complete, or if washing thawed raw semen, by density gradient centrifugation with ISolate ...

Q. Is it possible to add protein, such as Serum Substitute Supplement (SSS) or Human Serum Albumin to complete media? A. Our complete media contain the concentration of protein indicated below; it is possible to add more protein if needed, as long as you consider that the following has already been added. Multipurpose Handling Media-Complete (MHM-C) with Gentamicin is pre-supplemented with HSA for a final protein concentration of 5 mg/mL. Continuous Single Culture-NX Complete (CSCM-N...