Protocols for Stem Cell Expansion

Mesenchymal Stem Cell (MSC) Isolation

Adipose Tissue – Rapid Isolation
Adipose Tissue – Standard Isolation
Cord Blood
Umbilical Cord

Mesenchymal Stem Cell Expansion

Coating Culture Vessels
Thawing Mesenchymal Stem Cells
Expansion and Subculture of Human Mesenchymal Stem Cells

Mesenchymal Stem Cell Immune Modulation

Immune Modulation on T Cells Using Flow Cytometry and PBMCs

Neural Progenitor Cell (NPC) Expansion

Coating Culture Vessels
Thawing Neural Progenitor Cells
Expansion and Subculture of Human Neural Progenitor Cells
Harvesting Neurosphere Suspension Cultures

Cancer Stem Cell (CSC) Expansion and Tumorsphere Formation

Preparing Complete Medium
Thawing Cancer Stem Cells for Tumorsphere Formation
Plating Cancer Stem Cells for Tumorsphere Formation
Passaging Tumorspheres

Amniotic Fluid Stem Cells (AFSC) Expansion

Thawing Amniotic Fluid Stem Cells
Expansion and Subculture of Human Amniotic Fluid Stem Cells