Protocol for Amniotic Fluid Stem Cell Expansion
Amniotic fluid stem cells (AFSC) are clonogenic stem cells with the capacity to differentiate into cells of the endoderm, mesoderm, and ectoderm lineages. Derived from the amniotic fluid, AFSCs avert much of the controversy of human embryonic stem cells (hESCs) while offering the desired stem cell characteristics of self-renewal and pluripotency.
The following protocol outlines steps for amniotic fluid stem cell expansion using PRIME-XV® AFSC Expansion Medium. This medium is manufactured under cGMP conditions and comes completely formulated and ready for use. For more information on this medium, please refer to the product insert.
The following amniotic fluid stem cell expansion protocols can be found below:
These procedures are a general guideline for culturing human AFSCs derived from human amniocentesis. Procedures for optimal growth conditions should be determined for each application and cell type as appropriate. Safe laboratory procedures should be followed and protective clothing should be worn when handing this medium. The acute and chronic effects of over-exposure to this medium are unknown.
Human amniotic fluid stem cell expansion in PRIME-XV AFSC Expansion Medium after six passages showed positive staining of the pluripotency markers OCT-4A (top) and Nanog (bottom). Nuclei were counterstained with DAPI (blue).