Protocol for Neural Progenitor Cell Expansion
Neural progenitor cells (NPCs) are multipotent stem cells with the capability to differentiate into neurons and glial cells (oligodendrocytes and astrocytes). Therefore, successful in vitro neural progenitor cell expansion offers significant therapeutic potential for cell therapy applications.
This protocol outlines the steps needed to expand and maintain human-, mouse-, and rat-derived NPCs using PRIME-XV® NPC Expansion Xeno-free, Serum-Free Medium. PRIME-XV NPC Expansion XSFM is manufactured in cGMP conditions and comes completely formulated and ready for use. For more information on this medium, please refer to the product insert.
Human neural progenitor cells plated on PRIME-XV Human Fibronectin-coated plate and cultured in PRIME-XV NPC Expansion XSFM retained Nestin expression. Nuclei counterstained with DAPI (blue).
- Phosphate Buffered Solution (PBS) (Irvine Scientific, Catalog #9236)
PRIME-XV Human Fibronectin (Irvine Scientific, Catalog #31002) OR
PRIME-XV MatrlS F (Irvine Scientific, Catalog #31001)
- Culture vessel (T-25, T-75)
- PRIME-XV NPC Expansion XSFM (Irvine Scientific, Catalog #91131)
- Frozen vial of neural progenitor cells
- PBS without calcium and magnesium (Irvine Scientific, Catalog #9240)
- Dissociation Reagent (such as Accutase®)
The following neural progenitor cell expansion protocols can be found below:
- Coating Culture Vessels
- Thawing Neural Progenitor Cells
- Expansion and Subculture of Human Neural Progenitor Cells
- Harvesting Neurosphere Suspension Cultures
These procedures are general guidelines for culturing human neural progenitor cells isolated from fetal tissue or embryonic stem cells, as well as for murine neural progenitor cells isolated from fetuses or post-natal brains. Procedures for optimal growth conditions should be determined for each application and cell type as appropriate. Safe laboratory procedures should be followed and protective clothing should be worn when handing this medium. The acute and chronic effects of over-exposure to this medium are unknown.
Coating Culture Vessels
Thawing Neural Progenitor Cells
Expansion and Subculture Human Neural Progenitor Cells
Harvesting Neurosphere Suspension Cultures
Accutase® is a registered trademark of ICT inc.
Parafilm® is a registered trademark of Bemis Company, Inc.