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Y-27632, MF
This product is registered in the Drug Master File in Japan.
It is a selective and potent inhibitor of ROCK (Rho-associated coiled-coil forming kinase/Rho binding kinase).
Y-27632 has a variety of activities including vascular smooth muscle contraction through the ROCK signaling (Ki = 140 nmol/L p160ROCK). This product has been reported to suppress death of human ES cells and human iPS cells at the time of cell dispersion, and also improve viability of the cells after cryopreservation.
[ROCK : Rho-associated coiled-coil forming kinase]
This product is for research use only. Do not administer it to human.


Applications/Properties
For Cell Culture
Manufacturer: | FUJIFILM Wako Pure Chemical Corporation |
Specification Assay: | 98.0+% (HPLC) |
CAS RN: | 331752-47-7 |
Molecular Formula: | C14H21N3O・2HCl・H2O |
Molecular Weight: | 338.27 |
GHS: | ![]() |
Precautions for Use: | Packed on inactive gas |
Appearance: | White - pale yellow, crystalline powder - powder |
Boiling Point: | About 85°C |
Specific Rotation: | +2.0 - +10.0°(D/20°C)(c=1.0,CH3OH) |
Purity: | Bacterial endotoxins : <0.25 EU/mg |
Alias: | (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)cyclohexanecarboxamide Dihydrochloride Monohydrate |
Preparation:
Solubility Data for Y-27632 dihydrochloride
C14H21N3O・2HCl・H2O = 338.27
Solvent | Conc. mg/mL | Conc. mmol/L | |
Solubility | Water | 10 mg/mL | 29.56 mmol/L |
Ethanol | 10 mg/mL | 29.56 mmol/L |
Preparing Stock Solutions
C14H21N3O・2HCl・H2O = 338.27
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
1 mmol/L | 2.956 mL | 14.781 mL | 29.562 mL |
5 mmol/L | 0.591 mL | 2.956 mL | 5.912 mL |
10 mmol/L | 0.296 mL | 1.478 mL | 2.956 mL |
50 mmol/L | 0.059 mL | 0.296 mL | 0.591 mL |
Y-27632 Comparison Chart
ICH-Q7 | ISO9001 | Sterility test |
Viable count test |
Animal- derived- free |
Bacterial Endotoxin test |
Mycoplasma test |
Powder/ Liquid |
References* | |
Y-27632 (GMP-compliant)** | ✓ | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | - |
Y-27632, MF*** | - | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | Link |
CultureSure Y-27632 | - | ✓ | - | - | ✓ | ✓ | ✓ | Powder | Link |
CultureSure 10mmol/L Y-27632 Solution, Animal-derived-free |
- | ✓ | ✓ | - | ✓ | ✓ | ✓ |
Liquid Sterilized with filter |
Link |
* Click to move to the PubMed Central search results.
** This product is complied with ICH-Q7 (GMP for APIs) as a raw material for commercial production of regenerative medicine products.
*** The manufacturing process and analytical procedure are validated.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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Product content may differ from the actual image due to minor specification changes etc.
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If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.