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Recombinant Rat TNF-α

In stock
Catalog ID
300-18
Size Stock SKU Unit Price Qty
5 µg In Stock 300-18-5UG
$73.00
20 µg In Stock 300-18-20UG
$183.00
100 µg In Stock 300-18-100UG
$457.00
500 µg Inquire for Availability 300-18-500UG
$1,971.00
1 mg Inquire for Availability 300-18-1MG
$3,284.00
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Details

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF
Accession Number P16599
Source Genetically modified E.coli
Predicted Molecular Mass Monomer, 17.3 kDa (157 aa)
AA Sequence MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Formulation Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Product Reconstitution Sterile water at 0.1 mg/mL
Country of Origin USA

 

Specification* Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
Endotoxin Kinetic LAL ≤ 1 EU/µg
Biological Activity (ED50) Cytolysis of mouse L929 cells in the presence of Actinomycin D ≤ 0.05 ng/mL

 

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

TNF-alpha TNF alpha

Specifications

Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20°C to-80°C as supplied.
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