Recombinant Mouse LIF

In stock

Catalog ID

200-33

Size	Stock	SKU	Unit Price
5 µg	In Stock	200-33-5UG	$73.00
20 µg	In Stock	200-33-20UG	$183.00
100 µg	In Stock	200-33-100UG	$590.00
500 µg	In Stock	200-33-500UG	$1,825.00
1 mg	Inquire for Availability	200-33-1MG	$3,041.00
5 µg (Animal-Free)	Inquire for Availability	200-33AF-5UG	$79.00
20 µg (Animal-Free)	In Stock	200-33AF-20UG	$200.00
100 µg (Animal-Free)	Inquire for Availability	200-33AF-100UG	$651.00
500 µg (Animal-Free)	Inquire for Availability	200-33AF-500UG	$2,008.00
1 mg (Animal-Free)	Inquire for Availability	200-33AF-1MG	$3,346.00

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Details

Leukemia inhibitory factor (LIF) is a member of the interleukin 6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. Mouse LIF promotes mouse embryonic stem (ES) cell self-renewal and pluripotency in long-term cell culture systems, similar to the functional activity of FGF-basic in human ES cell culture systems.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	Leukocyte Inhibitory Factor
Accession Number	P09056
Source	Genetically modified E.coli
Predicted Molecular Mass	Monomer, 20 kDa (181 aa)
AA Sequence	MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F
Formulation	Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Product Reconstitution	Sterile 10 mM acetic acid at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	IL-6 production by M1 cells	≤ 1 ng/mL

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to-80°C as supplied.

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