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Recombinant Rat VEGF-165

In stock
Catalog ID
300-31
Size Stock SKU Unit Price Qty
10 µg In Stock 300-31-10UG
$183.00
10 µg (Animal-Free) Inquire for Availability 300-31AF-10UG
$200.00
100 µg In Stock 300-31-100UG
$529.00
100 µg (Animal-Free) Inquire for Availability 300-31AF-100UG
$577.00
500 µg In Stock 300-31-500UG
$2,627.00
500 µg (Animal-Free) Inquire for Availability 300-31AF-500UG
$2,889.00
1 mg Inquire for Availability 300-31-1MG
$4,379.00
1 mg (Animal-Free) Inquire for Availability 300-31AF-1MG
$4,817.00
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Details

Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names VEGF-A, VPF, glioma-derived endothelial cell mitogen
Accession Number P16612-2
Source Genetically modified E.coli
Predicted Molecular Mass Dimer, 19.4/38.7 kDa (165/330 aa)
AA Sequence MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Formulation Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5
Product Reconstitution Sterile water at 0.1 mg/mL
Country of Origin USA

 

Specification* Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
Endotoxin Kinetic LAL ≤ 0.1 EU/µg
Biological Activity (ED50) HUVEC proliferation ≤ 8 ng/mL

 

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20°C to-80°C as supplied.
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