Recombinant Mouse PDGF-BB
Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.
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Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
|Alternate Names||GDGF, ODGF|
|Source||Genetically modified E.coli|
|Predicted Molecular Mass||Dimer, 12.4/24.7 kDa (110/220 aa)|
|AA Sequence||MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT|
|Formulation||Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium citrate, pH 3.0|
|Product Reconstitution||Sterile water at 0.1 mg/mL|
|Country of Origin||USA|
|Specification*||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS PAGE||≥ 95%|
|Endotoxin||Kinetic LAL||≤ 1 EU/µg|
|Biological Activity (ED50)||3T3 cell proliferation||≤ 2 ng/mL ; ≥ 5.0 x 105 units/mg|
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.