Recombinant Mouse EGF
Epidermal growth factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR).
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
|Alternate Names||Urogastrone, URG|
|Source||Genetically modified E.coli|
|Predicted Molecular Mass||Monomer, 6.2 kDa (54 aa)|
|AA Sequence||MNSYPGCPSS YDGYCLNGGV CMHIESLDSY TCNCVIGYSG DRCQTRDLRW WELR|
|Formulation||Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5|
|Product Reconstitution||Sterile water at 0.1 mg/mL|
|Country of Origin||USA|
|Specification*||Method of Determination||Acceptance Criteria|
|Purity||Reducing and Non-Reducing SDS PAGE||≥ 95%|
|Endotoxin||Kinetic LAL||≤ 1 EU/µg|
|Biological Activity (ED50)||BALB/c 3T3 cell proliferation||≤ 0.25 ng/mL|
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.