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Recombinant Human SHH

In stock
Catalog ID
100-168
Size Stock SKU Unit Price Qty
5 µg Inquire for Availability 100-168-5UG
$73.00
    25 µg In Stock 100-168-25UG
    $183.00
      100 µg In Stock 100-168-100UG
      $457.00
        500 µg Inquire for Availability 100-168-500UG
        $1,825.00
          1 mg Inquire for Availability 100-168-1MG
          $3,041.00
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            Details

            Sonic hedgehog (SHH) is a member of a small group of hedgehog secreted proteins that are essential for development in both vertebrates and invertebrates. There are three mammalian hedgehog homologues, sonic, desert, and indian, that signal via the Patched-1 and Patched-2 receptors. SHH is a morphogen that is essential during vertebrate organogenesis and adult stem cell division.

            Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

            Instructions

            Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

            Technical Specifications

            Alternate Names Shh, HHG-1
            Accession Number Q15465
            Source Genetically modified E.coli
            Predicted Molecular Mass Monomer, 20.2 kDa (179 aa)
            AA Sequence MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGCFP
            Formulation Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5
            Product Reconstitution Sterile water at 0.1 mg/mL
            Country of Origin USA

             

            Specification* Method of Determination Acceptance Criteria
            Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
            Endotoxin Kinetic LAL ≤ 0.1 EU/µg
            Biological Activity (ED50) CCL-226 cell proliferation ≤ 5 ug/mL; ≥ 200 units/mg

             

            *Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

            Specifications

            Storage
            At or below -20°C
            Shelf Life
            12 months from date of receipt when stored at -20°C to -80°C as supplied.
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