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CTGrade rh IL-4

In stock
Catalog ID
500-05
Size Stock SKU Unit Price Qty
100 µg In Stock 500-05-100UG
$1,617.00
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Details

CTGrade rh IL-4 is a recombinant human protein that is produced from E. coli and is designed to support pre-clinical and clinical research, as well as commercial applications, and offers:

  • High biological activity verified by a relevant bioactivity assay
  • Low endotoxin levels 
  • ≥ 98% purity
  • High lot-to-lot consistency

Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.

IL-4 is an immunomodulatory cytokine that stimulates B cell proliferation and activates eosinophils, basophils, and mast cells.

  • Induces vascular cell adhesion molecule (VCAM)-1 expression on endothelial cells and involved in collagen production by fibroblasts
  • Plays a key role in promotion of Th2 differentiation from naïve helper T cells
  • Defines the Th2 phenotype of lymphocytes
  • Regulates cell proliferation, apoptosis, and expression of numerous genes in various cell types

The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

The CTGrade products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.

SDS-PAGE Showing a Prominent Band at 14.4 kDa

Figure 1. CTGrade rh IL-4 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14.4 kDa.* 

*Predicted MW is 15.1 kDa, but runs on SDS-PAGE at 14.4 kDa.

Cell-Based Proliferation Assay

Figure 2. The biological activity of CTGrade rh IL-4 was determined in TF-1 cell-based proliferation assay.

Technical Specifications

Alternate Names B-cell stimulatory factor-1, BSF-1, BCDF, BCGF
Accession Number P05112
AA Sequence MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Predicted Molecular Mass Monomer, 15.1 kDa (130 aa)
Formulation CTGrade rh IL-4 lyophilized at 1 mg/ml in 20 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered
Protein Purity ≥ 98% determined with Coomassie staining
Endotoxin < 0.05 EU/µg using USP <85> / EP 2.6.14
Bioactivity ED50 is determined by the dose-dependent TF-1 cell proliferation cells. The ED50 is typically less than 0.25 ng/mL. The international units of CTGrade rh IL-4 is approximately 1.4x104 IU/µg, which is calibrated against recombinant human interleukin 4 WHO International Standard (NIBSC code 88/656).
Quality Carrier-free and no animal- or human-derived materials were used during manufacturing

 

Specifications

Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
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