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CTGrade rh IL-10

In stock
Catalog ID
500-16
Size Stock SKU Unit Price Qty
50 µg Inquire for Availability 500-16-50UG
$1,401.00
100 µg In Stock 500-16-100UG
$1,961.00
1 mg Inquire for Availability 500-16-1MG
$7,372.00
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Details

CTGrade rh IL-10 is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:

  • High biological activity verified by a relevant bioactivity assay
  • Low endotoxin levels
  • ≥ 97% purity
  • High lot-to-lot consistency

Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.

IL-10 is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells.

  • Inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFNγ), tumor necrosis factor alpha (TNFα), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells
  • Suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells

The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

The CTGrade products are manufactured in a facility that does not use or process beta- lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.

SDS-PAGE Showing a Single Prominent Band at 18.8 kDa

Figure 1. CTGrade rh IL-10 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) conditions and visualized by InstantBlue staining showing a single prominent band at 18.8 kDa.

Cell-Based Proliferation Assay

Figure 2. The biological activity of CTGrade rh IL-10 was determined in a cell proliferation assay using MC/9 mouse mast cells.

Technical Specifications

Alternate Names B-TCGF, CSIF, TGIF
Accession Number P22301
AA Sequence MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Predicted Molecular Mass Non-covalent homodimer, 18.8 kDa (161aa)
Formulation CTGrade rh IL-10 lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered
Protein Purity ≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin < 0.05 EU/µg using USP <85> / EP 2.6.14
Bioactivity ED50 is determined by the dose-dependent proliferation of MC/9 cells. The ED50 is typically less than 5 ng/mL. The international units of CTGrade rh IL-10 is approximately 5.0 x 103 IU/µg, which is calibrated against recombinant human interleukin 10 WHO International Standard (NIBSC code 93/722).
Quality Carrier-free and no animal- or human-derived materials were used during manufacturing

 

*For research use or further manufacturing and in ex vivo cell therapy applications.

**Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.

***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
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