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CTGrade rh GM-CSF

In stock
Catalog ID
500-11
Size Stock SKU Unit Price Qty
100 µg In Stock 500-11-100UG
$1,981.00
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Details

CTGrade rh GM-CSF is a recombinant human protein that is produced from E. coli and is designed to support preclinical and clinical research, as well as commercial applications, and offers:

  • High biological activity verified by a relevant bioactivity assay
  • Low endotoxin levels 
  • ≥ 98% purity
  • High lot-to-lot consistency

Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.

GM-CSF is a hematopoietic growth factor with the ability to form colonies of granulocytes and macrophages. 

  • Enhances the number of circulating white blood cells and function of neutrophils and monocytes
  • Regulates multiple functions via transcription factors in the differentiated cells, including cell survival, proliferation, and maturation
  • Generates in vitro murine and human DC populations from bone marrow precursors and blood monocytes, respectively
  • Human and mouse GM-CSF do not show cross-reactivity

The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

The CTGrade products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.

SDS-PAGE Showing a Prominent Band at 14.6 kDa

Figure 1. CTGrade rh GM-CSF (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14.6 kDa.

Cell-Based Proliferation Assay

Figure 2. The biological activity of CTGrade rh GM-CSF was determined in TF-1 cell-based proliferation assay.

Technical Specifications

Alternate Names CSF-2, Pluripoietin-α, MGI-1GM
Accession Number P04141
AA Sequence MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Predicted Molecular Mass Monomer, 14.6 kDa (128 aa)
Formulation CTGrade rh GM-CSF lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered
Protein Purity ≥ 98% determined with Coomassie staining
Endotoxin < 0.02 EU/µg using USP <85> / EP 2.6.14
Bioactivity ED50 is determined by the dose-dependent proliferation of TF-1 cells. The ED50 is typically less than 0.2 ng/mL. The international units of CTGrade rh GM-CSF is approximately 1.6 x 104 IU/µg, which is calibrated against recombinant human granulocyte macrophage colony stimulating factor WHO International Standard (NIBSC code 88/646).
Quality Carrier-free and no animal- or human-derived materials were used during manufacturing

 

Specifications

Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
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