CTGrade rh FLT-3 Ligand
CTGrade rh FLT-3 Ligand is a recombinant protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:
- High biological activity verified by a relevant bioactivity assay
- Low endotoxin levels
- ≥ 97% purity
- High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
FLT-3 Ligand is a growth factor that regulates proliferation of early hematopoietic cells. FLT-3 Ligand binds to cells expressing the tyrosine kinase receptor FLT-3.
- FLT-3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation
- Unlike SCF, FLT-3 Ligand exerts no activity on mast cells. Multiple isoforms of FLT-3 Ligand have been identified
- The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 aa)
- The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform
The biological activity of CTGrade interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Single Prominent Band at 17.7 kDa
Figure 1. CTGrade rh FLT-3 Ligand (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 17.7 kDa.
Cell-Based Proliferation Assay
Figure 2. The biological activity of CTGrade rh FLT-3 Ligand was determined in a cell proliferation assay in OCI-AML5 cells.
|Alternate Names||Flt3L, Fms-like tyrosine kinase 3 ligand|
|AA Sequence||MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT|
|Predicted Molecular Mass||Monomer, 17.7 kDa (155aa)|
|Formulation||CTGrade rh FLT-3 Ligand lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5, 0.2 µm filtered|
|Protein Purity||≥ 97% determined by reducing and non-reducing SDS-PAGE analysis|
|Endotoxin||< 0.05 EU/µg using USP <85>/EP 2.6.14|
|Bioactivity||ED50 is determined by the dose-dependent Proliferation of OCI-AML5 cells. The ED50 is typically less than 5 ng/mL. The international units of CTGrade rh FLT-3 Ligand is approximately 1.6 x 103 IU/µg, which is calibrated against recombinant Human FLT-3 Ligand WHO International Standard (NIBSC code 96/532).|
|Quality||Carrier-free and no animal- or human-derived materials were used during manufacturing|
*For research use or further manufacturing and in ex vivo cell therapy applications.
**Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.
***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.