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CTGrade GMP rh IL-2(C126S)

In stock
Catalog ID
500-01
Size Stock SKU Unit Price Qty
50 µg In Stock 500-01-50UG
$410.00
    100 µg In Stock 500-01-100UG
    $764.00
      1 mg In Stock 500-01-1MG
      $2,730.00
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        Details

        CTGrade GMP rh IL-2C126S is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications, and offers:

        • The core amino acid sequence and 126 C to S sequence of CTGrade GMP rh IL-2 are identical to Proleukin
        • High biological activity verified by a relevant bioactivity assay
        • Low endotoxin levels
        • ≥ 97% purity
        • High lot-to-lot consistency

        Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.

        IL-2C126S is an immunomodulatory cytokine that is produced by lymphocytes. IL-2C126S signals through the IL-2R receptor to induce activated T cell proliferation and promote T cell differentiation.

        • Stimulates the proliferation and differentiation of B cells, natural killer (NK) cells, monocytes, and macrophages
        • Promotes proliferation and differentiation of CAR T cells, T cells, and NK cells
        • Activates and expands T cells and NK cells from peripheral blood

        The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

        The CTGrade GMP products are manufactured in a facility that does not use or process beta lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.

        SDS-PAGE Showing a Single Prominent Band at 14 kDa

        Figure 1. CTGrade GMP rh IL-2C126S (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14 kDa. Predicted MW is 15.5 kDa, but runs on SDS-PAGE at ~14kDa.

        Cell-Based Proliferation Assay

        Figure 2. The biological activity of CTGrade GMP rh IL-2C126S was determined in a CTLL-2 cell-based proliferation assay.

        Technical Specifications

        Alternate Names T cell growth factor, TCGF, Aldesleukin
        Accession Number P60568
        AA Sequence MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFSQSII STLT
        Predicted Molecular Mass Monomer, 15.5 kDa (134 aa)
        Formulation CTGrade GMP rh IL-2C126S lyophilized at 1 mg/ml in 0.1% Trifluoroacetic Acid (TFA), 0.2 µm filtered
        Protein Purity ≥ 97% determined by reducing and non-reducing SDS-PAGE analysis
        Endotoxin < 0.01 EU/µg using USP <85> / EP 2.6.14
        Bioactivity ED50 is determined by the dose-dependent proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade GMP rh IL-2C126S is approximately 1.8 x 104 IU/µg, which is calibrated against recombinant human interleukin 2 WHO International Standard (NIBSC code 86/500).
        Quality Carrier-free and no animal- or human-derived materials were used during manufacturing

         

        *Animal component-free to the tertiary level (no animal components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.

        **For research use or further manufacturing and in ex vivo cell therapy applications.

        ***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

        Specifications

        Storage
        This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
        Shelf Life
        Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
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