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Recombinant Mouse LIF

In stock
Catalog ID
200-33
Size Stock SKU Unit Price Qty
5 µg In Stock 200-33-5UG
$73.00
    20 µg In Stock 200-33-20UG
    $183.00
      100 µg In Stock 200-33-100UG
      $590.00
        500 µg In Stock 200-33-500UG
        $1,825.00
          1 mg Inquire for Availability 200-33-1MG
          $3,041.00
            5 µg (Animal-Free) In Stock 200-33AF-5UG
            $79.00
              20 µg (Animal-Free) In Stock 200-33AF-20UG
              $200.00
                100 µg (Animal-Free) In Stock 200-33AF-100UG
                $651.00
                  500 µg (Animal-Free) In Stock 200-33AF-500UG
                  $2,008.00
                    1 mg (Animal-Free) Inquire for Availability 200-33AF-1MG
                    $3,346.00
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                      Details

                      Leukemia inhibitory factor (LIF) is a member of the interleukin 6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. Mouse LIF promotes mouse embryonic stem (ES) cell self-renewal and pluripotency in long-term cell culture systems, similar to the functional activity of FGF-basic in human ES cell culture systems.

                      Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

                      Instructions

                      Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

                      Technical Specifications

                      Alternate Names Leukocyte Inhibitory Factor
                      Accession Number P09056
                      Source Genetically modified E.coli
                      Predicted Molecular Mass Monomer, 20 kDa (181 aa)
                      AA Sequence MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F
                      Formulation Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
                      Product Reconstitution Sterile 10 mM acetic acid at 0.1 mg/mL
                      Country of Origin USA

                       

                      Specification* Method of Determination Acceptance Criteria
                      Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
                      Endotoxin Kinetic LAL ≤ 0.1 EU/µg
                      Biological Activity (ED50) IL-6 production by M1 cells ≤ 1 ng/mL

                       

                      *Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

                      Specifications

                      Storage
                      At or below -20°C
                      Shelf Life
                      12 months from date of receipt when stored at -20°C to-80°C as supplied.
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