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Recombinant Human R-Spondin-1

In stock
Catalog ID
100-208
Size Stock SKU Unit Price Qty
5 µg Inquire for Availability 100-208-5UG
$73.00
    25 µg In Stock 100-208-25UG
    $183.00
      100 µg In Stock 100-208-100UG
      $584.00
        500 µg In Stock 100-208-500UG
        $1,825.00
          1 mg In Stock 100-208-1MG
          $3,649.00
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            Details

            R-Spondin 1 (RSPO-1) is a secreted growth factor that stabilizes β-catenin and enhances WNT signaling. RSPO-1 is expressed in many tissues and regulates female sex determination, XX gonad development, oocyte differentiation, and hematopoietic stem cell specification. The RSPO-1 stabilization of β-catenin promotes intestinal crypt cell proliferation. This suggests that RSPO-1 functions as a stem cell growth factor and may have therapeutic potential in the treatment of gastrointestinal diseases and chemotherapy-induced intestinal mucositis. Additionally, recombinant RSPO-1 is a critical protein used to establish 3D intestinal organoid cultures.

            Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

            Instructions

            Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

            Technical Specifications

            Alternate Names hRspo1, Roof plate-specific spondin-1
            Accession Number Q2MKA7
            Source Genetically modified CHO cells
            Predicted Molecular Mass Monomer, 26.8 kDa (243 aa)
            AA Sequence SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
            Formulation Lyophilized from a 0.2 µm filtered solution containing phosphate buffered saline (PBS)
            Product Reconstitution Sterile water at 0.1 mg/mL
            Country of Origin USA

             

            Specification* Method of Determination Acceptance Criteria
            Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
            Endotoxin Kinetic LAL (50% confidence) ≤ 0.1 EU/µg
            Biological Activity (ED50) Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a ≤ 50 ng/mL

             

            *Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

            Specifications

            Storage
            At or below -20°C
            Shelf Life
            12 months from date of receipt when stored at -20°C to -80°C as supplied.
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