IL-4 is a pleiotropic cytokine produced mainly by a subpopulation of activated T cells called T helper 2 (Th2). It is also produced by T helper 1 (Th1) cells and mast cells. Biological activities of IL-4 are mediated by specific receptors expressed in a variety of cell types (helper T cells, endothelial cells, mast cells and macrophages). IL-4 has a wide range of effects on both hematopoietic cells and non-hematopoietic cells, such as smooth muscle and epithelial cells. Human IL-4 share 50% sequence homology with murine IL-4, but its activities are species-specific; human IL-4 shows no activity on murine cells. Recombinant Human IL-4 is a non-glycosylated protein, containing 130 amino acids with a molecular weight of 15 kDa.
Accession Number: P05112
Synonyms: B cell Stimulating Factor, BSF-1, BCDF, BCGF
Purity: ≥ 95% purity. Determined by HPLC, reducing and non-reducing SDS-PAGE, and UV spectroscopy
Bioactivity: ED50 typically less than 0.5 ng/mL. ED50 determined by dose-dependent proliferation of human TF1 cells.