Recombinant Human FGF-basic 147 / FGF-2

In stock

Catalog ID

100-28

Size	Stock	SKU	Unit Price
10 µg	In Stock	100-28-10UG	$73.00
50 µg	In Stock	100-28-50UG	$183.00
100 µg	In Stock	100-28-100UG	$330.00
500 µg	In Stock	100-28-500UG	$839.00
1 mg	In Stock	100-28-1MG	$1,460.00
10 µg (Animal-Free)	In Stock	100-28AF-10UG	$79.00
50 µg (Animal-Free)	In Stock	100-28AF-50UG	$200.00
100 µg (Animal-Free)	In Stock	100-28AF-100UG	$359.00
500 µg (Animal-Free)	In Stock	100-28AF-500UG	$925.00
1 mg (Animal-Free)	In Stock	100-28AF-1MG	$1,607.00

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Details

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degradation of the full length FGF-basic N-terminus results in a truncated FGF-basic 147aa protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-basic within the cell, but do not affect the biological activity of FGF-basic. Therefore, there are no detectable differences in biological activity between the full length FGF-basic 154aa and the truncated FGF-basic 147aa recombinant proteins.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names	FGF2, HBGF-2, Prostatropin
Accession Number	P09038
Source	Genetically modified E.coli
Predicted Molecular Mass	Monomer, 16.5 kDa (147 aa)
AA Sequence	MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Formulation	Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Product Reconstitution	Sterile water at 0.1 mg/mL
Country of Origin	USA

Specification*	Method of Determination	Acceptance Criteria
Purity	Reducing and Non-Reducing SDS PAGE	≥ 95%
Endotoxin	Kinetic LAL	≤ 0.1 EU/µg
Biological Activity (ED₅₀)	NR6R-3T3 cell proliferation	≤ 5 ng/mL; ≥ 2.0 x 10⁵ units/mg

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage

At or below -20°C

Shelf Life

12 months from date of receipt when stored at -20°C to-80°C as supplied.

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