We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Recombinant Human FGF-5
Fibroblast growth factor 5 (FGF-5) is a secreted heparin-binding growth factor that binds to FGF receptors 1 and 2 (FGFR1 and FGFR2). FGF-5 is expressed in the mesenchyme, skeletal muscles, central nervous system, and hair follicles to promote cell differentiation and proliferation. FGF-5 functions as a regulatory factor during hair elongation and skeletal muscle development.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
Alternate Names | Heparin-binding growth factor 5, HBGF-5, Smag-82 |
Accession Number | P12034 |
Source | Genetically modified E.coli |
Predicted Molecular Mass | Monomer, 27.7 kDa (252 aa) |
AA Sequence | MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG |
Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate and 100 mM sodium chloride, pH 7.5 |
Product Reconstitution | Sterile water at 0.1 mg/mL |
Country of Origin | USA |
Specification* | Method of Determination | Acceptance Criteria |
Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
Endotoxin | Kinetic LAL | ≤ 1 EU/µg |
Biological Activity (ED50) | NR6R-3T3 Proliferation with 1 µg heparin | ≤ 10 ng/mL; ≥ 1.0 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.