IL-2 is a T cell stimulatory cytokine best known for inducing activated T cell proliferation in vitro. IL-2 also stimulates proliferation and differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 is critical for the development, survival, and function of CD4+CD25+ regulatory T cells, which promotes T cell tolerance by suppressing T cell responses to self-antigens in vivo, thus inhibiting immune responses and preventing autoimmune diseases. IL-2 plays an essential role in sensitizing T cells to activation-induced cell death, a process mediated primarily by Fas and TNF signals to limit the magnitude of T cell expansion through programmed death of activated T cells. IL-2 binds to and signals through membrane receptor IL-2R, which is expressed almost exclusively by activated T-cells. IL-2R is a trimeric receptor complex consisting of three distinct subunits designated as IL-2Rα (CD25), IL-2Rβ (CD122), and common γ-chain (γc, CD132). IL-2Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex, including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-15. The common γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21. Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5 and the PI3K/Akt pathways (2-5). Recombinant Human IL-2 ACF has a serine substitute for cysteine at position 126. Recombinant Human IL-2 ACF is a non-glycosylated protein, containing 134 amino acids, with a molecular weight of 15.5 kDa.
- Accession Number: P60568
- Synonyms: T cell growth factor, TCGF, Aldesleukin
- Purity: ≥90% determined by reducing and non-reducing SDS-PAGE analysis
- Bioactivity: The ED50 is typically less than 1 ng/mL. ED50 is determined by the dose-dependent proliferation of CD3+ T cells.
- Animal component-free and carrier-free
- Custom packaging available
For research or further manufacturing use only.
Also available in other sizes.