Orexin A ELISA Kit Wako Data

Performance

Standard curve range	4.69～300 pg/mL
Samples	human cerebrospinal fluid, rat cerebrospinal fluid, rat plasma, rat serum^※１
Necessary amount of sample	25 μL
Duration of measurement	About 20 hours
Within-run reproducibility	CV＜5 ％
Between-run reproducibility	CV＜16 ％

※１: The levels of orexin A in normal human plasma and serum, as well as in wild-type mouse plasma and serum, are undetectable using the kit. The level may be high enough to be detected if orexin A was added or Orexin A was overexpressed.

Standard Curve

Examples of the measurement

Spike-and-recovery

	No spike	+ 5 pg/mL orexin A		+ 20 pg/mL orexin A		+ 100 pg/mL orexin A
	Measured (pg/mL)	Measured (pg/mL)	Recovery	Measured (pg/mL)	Recovery	Measured (pg/mL)	Recovery
Rat	51.82	54.96	96.7%	63.74	88.8%	120.77	79.6%
Cerebrospinal fluid 1
Rat	47.69	55.55	105.4%	63.40	93.7%	109.95	74.5%
Cerebrospinal fluid 2
Rat	38.05	43.99	102.2%	59.12	101.8%	101.16	73.3%
Cerebrospinal fluid 3
Human	12.25	14.29	82.8%	23.25	72.1%	65.16	58.1%
Cerebrospinal fluid 1
Human	47.21	50.45	96.6%	55.71	82.9%	107.12	72.8%
Cerebrospinal fluid 2
Human	24.41	25.99	88.4%	35.07	79.0%	83.51	67.1%
Cerebrospinal fluid 3
Rat	Not detected	5.64	112.8%	19.94	99.7%	89.58	89.6%
Serum 1
Rat	6.42	12.26	107.4%	25.05	94.8%	93.60	88.0%
Serum 2
Rat	1.45	6.81	105.6%	20.30	94.6%	91.13	89.8%
Plasma 1
Rat	2.28	7.20	98.9%	21.64	97.1%	90.51	88.5%
Plasma 2

These assays demonstrated that the kit is highly quantitative for human and rat samples.