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microRNA Extractor SP Kit Technical Information
Overview
Protocol Flow
This kit is for extraction of microRNA from serum or plasma of humans and animals. It enables microRNA extraction without use of phenol, chloroform, and other hazardous reagents required in previous methods.
Note
The following necessary reagents are not included.
1-Butanol (code No.022-16035)
2-Propanol (code No.168-21675)
Ethanol (code No.054-07225)
For plasma coagulation, please use EDTA or citric acid. Using heparin inhibit PCR detection after RNA extraction.
Application Data
Extraction from normal human plasma
Higher extraction rates observed than from previous methods.
Extraction from rat plasma and serum
Extraction from animal experiments possible.
Recovery rate and reproducibility
High reproducibility exhibited.
