Mature BDNF ELISA Kit Wako, High Sensitive

for Immunochemistry

Brain-derived neurotrophic factor (BDNF), which belongs to the nerve growth factor (NGF) family, is involved in neurogenesis, synaptogenesis, long-term potentiation etc. and is considered as a candidate marker for psychiatric diseases, including depression.
proBDNF, the precursor of BDNF, is processed to yield mature BDNF (mBDNF). Since proBDNF and mBDNF show completely different effects, there is a need for a method that can accurately measure mBDNF.
Mature BDNF ELISA Kit Wako, High Sensitive is a sandwich ELISA that combines anti mBDNF N-terminal end-specific monoclonal antibody and anti-BDNF monoclonal antibody. The cross-reactivity with proBDNF is substantially reduced by using end-specific antibodies, and the sensitivity is increased through use of a luminescent substrate. This kit can be used for detection mBDNF in human saliva and mouse serum or plasma.

• Specifically detects mBDNF
  - Reactivity with human proBDNF: 1.30%
• Highly sensitive
  - lower limit of calibration curve: 0.116 pg/mL

NOTE: 290-85801 Mature BDNF ELISA Kit Wako, High Sensitive, is an upgraded kit and replaces 298-83901 Mature BDNF Kit Wako, High Sensitive.

1. Want, A., Morgan, J. E. and Barde, Y. A.: Sci. Rep., 13(1), 7740(2023).
   Brain-derived neurotrophic factor measurements in mouse serum and plasma using a sensitive and specific enzyme-linked immunosorbent assay
2. Want, A. et al.: Brain Commun., 5(2), fcad046 (2023).
   Brain-derived neurotrophic factor released from blood platelets prevents dendritic atrophy of lesioned adult central nervous system neurons
3. Ikenouchi, A. et al. : Brain Behav.,e3278(2023).
   Association between salivarymature brain-derived neurotrophic factor and psychological distress in healthcare workers

Note: 1) - 3) use the predecessor of this kit, Mature BDNF ELISA Kit Wako, High Sensitive (Code No. 298-83901, discontinued) is used.

Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific 

• For research use or further manufacturing use only. Not for use in diagnostic procedures. 
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