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MagCapture microRNA Isolation Kit, Mouse Ago2 Technical Information
Recovering microRNA from mouse cell strains
We used MagCapture microRNA Isolation Kit, Mouse Ago2 and a previous product to obtain microRNA from mouse P388D1 cells (2×107 cells), then compared quantities of microRNA obtained with denaturing polyacrylamide gel electrophoresis (silver staining) (Fig. 1) and quantitative PCR (miR92a quantity) (Fig. 2). Results showed that the two products yielded equivalent quantities of microRNA.
Recovering free Ago2-bound microRNA from rat plasma
We used MagCapture microRNA Isolation Kit, Mouse Ago2 and a previous product to obtain microRNA from 200 μL of rat plasma, then compared quantities obtained with Quantitative PCR (miR92a quantity). As shown below, using the spin column method with the MagCapture resulted in approximately equivalent yield to our previous product, while the one-step method demonstrated greater quantities of microRNA recovered compared to our previous product.