We used MagCapture™ microRNA Isolation Kit, Human Ago2 and a previous product to obtain microRNA from human K562 cells (1×10⁷ cells), then compared quantities of microRNA obtained with denaturing polyacrylamide gel electrophoresis (silver staining) (Fig. 1) and quantitative PCR (miR-92a quantity) (Fig. 2). Results showed that the two products yielded equivalent quantities of microRNA.

We used MagCapture microRNA Isolation Kit, Human Ago2 and a previous product to obtain microRNA from pooled plasma of healthy individuals, then compared quantities obtained with quantitative PCR (miR-92a quantity). As shown below, using the spin column method with the MagCapture resulted in approximately equivalent yield to our previous product, while the one-step method demonstrated greater quantities of microRNA recovered compared to our previous product.