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Lysyl Endopeptidase, Mass Spectrometry Grade (Lys-C)
In stock
Catalog ID
125-05061, 121-05063
Details
for Proteome Research
Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained. Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.
Originally, the source of this product was indicated as "Achromobacter lyticus" based on the physiological and morphological properties of the bacteria. However, we confirmed that the 16SrDNA sequence was highly homologous to that of Lysobacter.
This product is for research use only. Do not administer it to humans. (BIOCHEMISTRY)
| Manufacturer: | FUJIFILM Wako Pure Chemical Corporation |
| Appearance: | Lyophilized form containing ca. 10% Tris-HCl buffer, pH 8 |
| Activity: | 0.03 - 0.07AU/vial |
| Molecular Weight: | 27,000 (gel filtration); 30,000 (SDS electrophoresis) |
| Solubility: | Soluble in water or buffer solution. |
| Stability: | Stable at 4°C, when dissolved in buffer of pH 5~12. Stable at 30°C in the range of pH 6~11, but unstable at 50°C or higher. |
| Optimal pH: | 9.0 -9.5 (Amidase activity) |
| Isoelectric point: | 6.9~7.0 |
| Substrate specificity: | Hydrolysable substrate ... Tos-Lys-Ome, Bz-Lys-NH2, Bz-Lys-pNA, Lys-pNA Unhydrolysable substrate ... Bz-Arg-NH2, Bz-Arg-pNa, Arg-pNA |
| Inhibitors: | DFP, PMSF, TLCK |
| Source: | Bacteria |
Features
- High specificity and efficiency of protein digestion allow for easy database searches by peptide mass.
- Improved cleavage at lysine residue and increase in the number of peptides are obtained by combination with trypsin.
- Packed in very small quantities according to the amounts used so that sufficient activity for in-gel digestion may be retained.
References
- Wada, Y., and Kadoya, M.: J. Mass Spectrom., 38, 117 (2003).
- Shevchenko, A., Wilm, MM., Vorm, O., and Mann., M.: Anal. Chem., 68, 850 (1996).
Alias
Lys-C
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific
- For research use only. Not for use in diagnostic procedures.
- Product content may differ from the actual image due to minor specification changes etc.
- If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
Specifications
Storage
Keep at -20°C
Data & Technical Information
Product Specifications
